Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).
SDS-PAGE Analysis of Purified GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).
Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S Recombinant Rabbit Anti-GFAP (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.