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Home >> Antibodies >> FITC (Fluorescein Isothiocyanate)

FITC (Fluorescein Isothiocyanate)

Mouse Monoclonal Antibody [Clone SPM395]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00

Product Details

Synonyms

Fluorescein isothiocyanate, FITC

Positive Control

Fluoresceinated proteins in solution or on tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Confocal Microscopy
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
In situ Nucleic Acid Hybridization
Western Blot
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.

Immunogen

Fluorescein isothiocyanate (FITC) conjugated to KLH

Cellular Localization

Depends on the target

Species Reactivity

All species.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

389.4Da (Molecular Formula: C21H11NO5S)

Specificity & Comments

This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Balogh P, et. al. Journal of Immunological Methods, 1994, 169(1):35-40.

Bioinformatics

NBT01277