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Home >> Antibodies >> Ferritin, Heavy Chain (FTH) (Microglia Marker)

Ferritin, Heavy Chain (FTH) (Microglia Marker)

Mouse Monoclonal Antibody [Clone FTH/2081]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Western Blot of HeLa, cell lysate using Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2081).

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Formalin-fixed, paraffin-embedded human pancreas stained with Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2081). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human pancreas stained with Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2081). Confirmation of Purity and Integrity of Antibody.

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SDS-PAGE Analysis Purified Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2081). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; FHC; FRIH; FTH1; FTHL6; Iron overload autosomal dominant; PIG15; Placenta immunoregulatory factor; Proliferation inducing gene 15 protein (PLIF)

Positive Control

HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

21kDa

Specificity & Comments

Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Y. Kaneko, T. Kitamoto, J. Tateishi, K. Yamaguchi. Ferritin immunohistochemistry as a marker for microglia. Acta Neuropathologica. 1989, Volume 79; 129-136.

Bioinformatics

  •  Entrez Gene ID: 2495
  •  Gene Symbol: FTH1
  •  SwissProt: P02794
  •  Chromosome Location: 11q12.3
  •  Unigene: 524910, 645560
NBT02081