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Home >> Antibodies >> FAT1 (FAT atypical cadherin 1)

FAT1 (FAT atypical cadherin 1)

Mouse Monoclonal Antibody [Clone FAT1-3D7/1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified FAT Mouse Monoclonal Antibody (FAT1-3D7/1). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Cadherin family member 7 precursor (CDHF7); Cadherin ME5; Cadherin related tumor suppressor homolog precursor (FAT protein homolog); FAT tumor suppressor homolog 1; hFat 1; Homolog of Drosophila tumor suppressor FAT precursor; nuclear form; Protein fat homolog; Protocadherin Fat 1

Positive Control

Wild type imaginal discs from third instar Drosophila larvae.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IF         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Cytoplasmic domain of Drosophila Fat protein.

Cellular Localization

Cell membrane. Nucleus. Cytoplasm > perinuclear region.

Species Reactivity

Drosophila melanogaster. Expected to cross-react with Chimpanzee. Rat (92%. 85% identity with immunogen. respectively) due to sequence homology.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

500kDa

Specificity & Comments

The FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychic disorders, and its action may be of patho-physiological importance.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Maddox, J. and Arnold, N.W. 1980. Prevention of industrial accidents. Med. J. Aust. 2: 484.

Bioinformatics

  •  Entrez Gene ID: 2195
  •  Gene Symbol: FAT1
  •  SwissProt: Q14517
  •  Chromosome Location: 4q35.2
  •  Unigene: 481371
NBT02322