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Home >> Antibodies >> Fascin-1 (Reed-Sternberg Cell Marker)

Fascin-1 (Reed-Sternberg Cell Marker)

Mouse Monoclonal Antibody [Clone FSCN1/416]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Fascin-1 Mouse Monoclonal Antibody (FSCN1/416).

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SDS-PAGE Analysis Purified Fascin-1 Mouse Monoclonal Antibody (FSCN1/416). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of HeLa cell lysate using Fascin-1 Mouse Monoclonal Antibody (FSCN1/416).

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Confocal immunofluorescence image of HeLa cells using Fascin-1 Mouse Monoclonal Antibody (FSCN1/416). Green (CF488) and Reddot is used to label the nuclei Red.

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Flow Cytometric Analysis of PFA-fixed K562 cells using Fascin-1 Mouse Monoclonal Antibody (FSCN1/416) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Product Details

Synonyms

55kDa actin-bundling protein; FAN1; Fascin homolog 1; Fascin1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); SNL

Positive Control

K562, HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Full length recombinant human FSCN1 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yamashiro-Matsumura S and Matsumura F. J Biol Chem 1985; 260(8): 5087.
  2. Yamashro-Matsumura S and Matsumura F. J Cell Biol 1986; 103:631.
  3. Duh F-M, et al. DNA Cell Biol 1994; 13(8):821.

Bioinformatics

  •  Entrez Gene ID: 6624
  •  Gene Symbol: FSCN1
  •  SwissProt: Q16658
  •  Chromosome Location: 7p22.1
  •  Unigene: 118400
NBT00416