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Home >> Antibodies >> Fas Ligand (FASLG)

Fas Ligand (FASLG)

Mouse Monoclonal Antibody [Clone FASLG/4456]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified Fas Ligand (FASLG) Mouse Monoclonal Antibody (FASLG/4456). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human lymph node stained with Fas Ligand (FASLG)Mouse Monoclonal Antibody (FASLG/4456). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Fas Ligand (FASLG) Monospecific Mouse Monoclonal Antibody (FASLG/4456).

Product Details

Synonyms

ALPS1B; Apoptosis (APO 1) antigen ligand 1; APT1LG1; APTL; CD178 antigen; CD95 ligand; Generalized lymphoproliferative disease; Gld; soluble form; TNFSF6; Tumor necrosis factor (ligand) superfamily member 6

Positive Control

Human tonsil or prostate.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human recombinant FASLG protein fragment (around aa107-222) (exact sequence is proprietary)

Cellular Localization

Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

26kDa (soluble FAS-L) / 40kDa (FAS-L membrane)

Specificity & Comments

Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an importantcomponent of specific effector mechanisms in immuno-surveillance againstvirus-infected or transformed cells. Two mechanisms appear to account forthis activity, one of which is the perforin-based process. Independently, aFAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surfaceglycoprotein that belongs to a family of receptors that includes CD40, nervegrowth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of whichundergo apoptosis in response to treatment with antibody to FAS. Thesefindings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FASmay be critically involved in CTL-mediated cytotoxicity.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Henkart, P.A. 1985. Mechanism of lymphocyte-mediated cytotoxicity.Annu. Rev. Immunol. 3: 31-58.

Bioinformatics

  •  Entrez Gene ID: 356
  •  Gene Symbol: FASLG
  •  SwissProt: P48023
  •  Chromosome Location: 1q24.3
  •  Unigene: 2007