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Home >> Antibodies >> Fas (TNFRSF6) associated factor 1

Fas (TNFRSF6) associated factor 1

Mouse Monoclonal Antibody [Clone CPTC-FAF1-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Cancer stained with FAF1 Mouse Monoclonal Antibody (CPTC-FAF1-2).

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SDS-PAGE Analysis Purified FAF1 Mouse Monoclonal Antibody (CPTC-FAF1-2). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with FAF1 Mouse Monoclonal Antibody (CPTC-FAF1-2).

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Western Blot Analysis of HEK293, HeLa, MCF-7 cell lysates using Purified FAF1 Mouse Monoclonal Antibody (CPTC-FAF1-2).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Fas (TNFRSF6) associated factor 1 Monoclonal Antibody (CPTC-FAF1-2).

Product Details

Synonyms

CGI-03; FAS-associated factor 1; HFAF1s; UBX domain-containing protein 12; UBX domain-containing protein 3A

Positive Control

HEK293, MCF-7, HeLa whole cell lysate. Human testis, prostate, breast carcinoma or colon carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length FAF1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

75-80kDa

Specificity & Comments

In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Nagata, S. and Golstein, P. 1995. The FAS death factor. Science 267: 1449-1456

Bioinformatics

  •  Entrez Gene ID: 11124
  •  Gene Symbol: FAF1
  •  SwissProt: Q9UNN5
  •  Chromosome Location: 1p32.3
  •  Unigene: 530402
NBT02268