2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Mouse Monoclonal Antibody [Clone ER506]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506).

img

Formalin-fixed, paraffin-embedded human Endometrium stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506).

img

Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506).

img

SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506). Confirmation of Integrity and Purity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor, alpha Mouse Monoclonal Antibody (ER506).

Product Details

Synonyms

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1

Positive Control

MCF-7 cells. Breast or Endometrial Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~67kDa

Specificity & Comments

This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zafrani B, et. al. Histopathology 2000; 37(6), 536-545.
  2. Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 1474-1481.

Bioinformatics

  •  Entrez Gene ID: 2099
  •  Gene Symbol: ESR1
  •  SwissProt: P03372
  •  Chromosome Location: 6q25.1
  •  Unigene: 208124
NBT00080