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Home >> Antibodies >> ERG (Vascular Endothelial & Prostate Marker)

ERG (Vascular Endothelial & Prostate Marker)

Recombinant Mouse Monoclonal Antibody [Clone rERG/6843]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human prostate tissue stained with ERG Recombinant Mouse Monoclonal Antibody (rERG/6843) at 2ug/ml. HIER: Tris/EDTA pH 9; 95°C/45min. 2°Ab: HRP-Polymer:30mins; DAB: 5min.

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IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rERG/6483 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified ERG Recombinant Mouse Monoclonal Antibody (rERG/6843). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ERG; ERG/EWS fusion gene; ERG/FUS fusion gene; ERG/TMPSSR2 fusion gene; ERG1; ETS-related; KCNH2; Oncogene ERG; p55; TMPRSS2/ERG fusion

Positive Control

Human tonsil, Ewing sarcoma or prostate adenocarcinoma tissues.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa450 to 479) (C-terminus) of human ERG (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

ERG (ETS-related gene) is a proto-oncogene, a member of the ETS family of transcription factors. The ERG gene encodes for a nuclear protein, also called ERG, which is involved in hematopoietic and endothelial development. ERG remains constitually expressed in endothelial cells in blood and lymphatic vessels, and in bone marrow stem cells. ERG is expressed in virtually all endothelial neoplasms including hemangioendothelioma, angiosarcoma and Kaposi sarcoma. ERG is overexpressed secondary to gene rearrangement in cases of prostate adenocarcinoma, gastrointestinal stromal tumor, synovial sarcoma, meningioma, epithelioid sarcoma, malignant rhabdoid tumor, acute myeloid leukemia and blastic extramedullary myeloid tumor, and rarely Ewing sarcoma / primitive peripheral neuroectodermal tumor, chondrosarcoma, osteosarcoma, and rhabdomyosarcoma. For the identification of endothelial differentiation ERG seems more sensitive and specific than any other marker. Moreover, the interpretation is often easier due to the nuclear reaction, which also allows for double stains with cytoplasmic markers like podoplanin. Among carcinomas, ERG is highly specific for prostate, while the sensitivity is moderate.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Miettinen M. Histopathology. 2014 Jan;64(1):101-18.
  2. Stockman DL, Mod Pathol. 2014 Apr;27(4):496-501.

Bioinformatics

  •  Entrez Gene ID: 2078
  •  Gene Symbol: ERG
  •  SwissProt: P11308
  •  Chromosome Location: 21q22.2
  •  Unigene: 473819