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Home >> Antibodies >> ER-beta-1 (Estrogen Receptor beta-1)

ER-beta-1 (Estrogen Receptor beta-1)

Mouse Monoclonal Antibody [Clone ERb455]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to PE

Price (USD)

429.00
img

Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ERb455).

Product Details

Synonyms

Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4

Positive Control

MCF-7 cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100 for 30 minutes at RT)
Optimal dilution for a specific application should be determined.

Immunogen

C-terminus fragment of recombinant human estrogen receptor beta protein

Cellular Localization

Predominantly nuclear

Species Reactivity

Human. Monkey. Mouse. Rat. Pig. Horse. Sheep.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

53-59kDa

Specificity & Comments

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

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Key References

  1. Skliris GP et. al. J Pathol 2002;197:155-62.

Bioinformatics

  •  Entrez Gene ID: 2100
  •  Gene Symbol: ESR2
  •  SwissProt: Q92731
  •  Chromosome Location: 14q23.2
  •  Unigene: 660607
NBT00455