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Home >> Antibodies >> ER-beta-1 (Estrogen Receptor beta-1)

ER-beta-1 (Estrogen Receptor beta-1)

Mouse Monoclonal Antibody [Clone ERb455]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Mouse Monoclonal Antibody (ERb455).

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Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Mouse Monoclonal Antibody (ERb455).

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SDS-PAGE Analysis Purified Estrogen Receptor beta1 Mouse Monoclonal Antibody (ERb455). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of human MCF-7 cell lysate using ER-beta1 Mouse Monoclonal Antibody (ERb455).

Product Details

Synonyms

Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4

Positive Control

MCF-7 cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

C-terminus fragment of recombinant human estrogen receptor beta protein

Cellular Localization

Predominantly nuclear

Species Reactivity

Human. Monkey. Mouse. Rat. Pig. Horse. Sheep.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

53-59kDa

Specificity & Comments

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Skliris GP et. al. J Pathol 2002;197:155-62.

Bioinformatics

  •  Entrez Gene ID: 2100
  •  Gene Symbol: ESR2
  •  SwissProt: Q92731
  •  Chromosome Location: 14q23.2
  •  Unigene: 660607
NBT00455