Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Ep-CAM Mouse Monoclonal Antibody (VU-1D9).
Surface staining of Human Colon HT29 cells using Ep-CAM Mouse Monoclonal Antibody (VU-1D9) (red) and isotype control antibody (green).
SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (VU-1D9).Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of MCF-7 cells EpCAM Mouse Monoclonal Antibody (VU-1D9) labeled with CF488 (green); NucSpot is used to label the nuclei (red).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (VU-1D9) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.