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Home >> Antibodies >> Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Mouse Monoclonal Antibody [Clone SPM528]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Ep-CAM Ab (SPM528).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1

Positive Control

HT29 cells. Breast tumor.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (5-10ul/million cells)
Immunofluorescence (1:50-1:100)
Western Blot (1:100-1:200)
Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 min at RT)(Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)Optimal dilution for a specific application should be determined.

Immunogen

Neuraminidase treated GLS-1 human small cell lung carcinoma cells

Cellular Localization

Cell surface

Species Reactivity

Human. Does not react with Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Litvinov SV et. al. J Biol Chem 125:437-446 (1994).2. Imrich S et. al. Cell Adhes Migr 6:30-38 (2012).3. Spizzo G et. al. J Clin Pathol 64:415-420 (2011).

Bioinformatics

  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2q21
  •  Unigene: 542050
NBT00380