Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Ep-CAM Mouse Monoclonal Antibody (SPM134).
Immunofluorescence Analysis of MeOH fixed MCF-7 cells labeling Ep-CAM with Ep-CAM Mouse Monoclonal Antibody (SPM134) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).
Flow Cytometric Analysis of PFA fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (SPM134) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.