Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with EpCAM Mouse Recombinant Monoclonal Antibody (rVU-1D9).
SDS-PAGE Analysis Purified EpCAM Mouse Recombinant Monoclonal Antibody (rVU-1D9). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of PFA fixed MCF-7 cells using EpCAM Mouse Recombinant Monoclonal Antibody (rVU-1D9) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Flow cytometric Analysis of live MCF-7 cells unstained (gray) or stained with CF488A-labeled Ep-CAM monoclonal antibody (rVU-1D9) (green)
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.