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Home >> Antibodies >> Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rVU-1D9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with EpCAM Mouse Recombinant Monoclonal Antibody (rVU-1D9).

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SDS-PAGE Analysis Purified EpCAM Mouse Recombinant Monoclonal Antibody (rVU-1D9). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of PFA fixed MCF-7 cells using EpCAM Mouse Recombinant Monoclonal Antibody (rVU-1D9) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Positive Control

MCF-7 or HT29 cells. Breast tumor.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human EpCAM protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Does not react with Rat or Ferret.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Related Products



200ug/ml of recombinant MAb Purified Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
  2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.

Bioinformatics

  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2p21
  •  Unigene: 542050
NBT01820