Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with EpCAM Mouse Monoclonal Antibody (EGP40/826).
SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (EGP40/826). Confirmation of Purity and Integrity of Antibody.
Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Mouse Monoclonal Antibody (EGP40/826) (Green). DyLight 554 Phalloidin labeled F-actin filaments (Red). DAPI stained nuclei (blue).
Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Mouse MAb (IgG1) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)
Flow Cytometric Analysis of PFA fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (EGP40/826) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.