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Home >> Antibodies >> Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone EGP40/2041R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R).

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Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R).

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SDS-PAGE Analysis Purified EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R).

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Western Blot of MCF-7 cell lysate using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R).

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Western Blot of human lung lysate using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Positive Control

MCF-7 or HT29 cells. Breast tumor.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Related Products



200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bjork, P., Jonsson, U., Svedberg, H., Larsson, K., Lind, P., Dillner, J., Hedlund, G., Dohlsten, M. and Kalland, T. 1993. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glyco- protein recognized by the C215 Mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241.

Bioinformatics

  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2q21
  •  Unigene: 542050
NBT02041