Home >> Antibodies >> EpCAM / CD326 (Epithelial Marker)

EpCAM / CD326 (Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone EGP40/1555R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

459.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of trypsinized-MCF-7 cells. EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of trypsinized-MCF-7 cells. EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Positive Control

MCF-7, HT29 cells or breast tumor

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human TACSTD1 protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Does not react with Dog, Mouse, Cat or Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.