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Home >> Antibodies >> Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Mouse Monoclonal Antibody [Clone EGP40/1372]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human colorectal carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372).

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Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372).

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Formalin-fixed, paraffin-embedded canine (dog) bladder stained with Ep-CAM Mouse Monoclonal Antibody (EGP40/1372).

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Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with Ep-CAM Mouse Monoclonal Antibody (EGP40/1372).

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Western blot analysis of human lung tissue lysate using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372).

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Western blot analysis of 293, A431 and HCT116 cell lysates using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372).

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SDS-PAGE Analysis Purified EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). Confirmation of Integrity and Purity of Antibody.

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Confocal Immunofluorescence of human colorectal carcinoma. EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) labeled with CF488 (green); Nuclei are labeled with Reddot (red).

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Immunofluorescence analysis of MCF-7 cells. EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) labeled with CF488 (green). Nuclei are stained with RedDot (red).

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Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372)

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Positive Control

MCF-7 or HT29 cells. Human lung or ovarian carcinoma (IHC).

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human. Dog. Cat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
  2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.
  3. Formalin-fixed, paraffin-embedded human colorectal carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372).

Bioinformatics

  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2p21
  •  Unigene: 542050