Home >> Antibodies >> EpCAM / CD326 (Epithelial Marker)

EpCAM / CD326 (Epithelial Marker)

Mouse Monoclonal Antibody [Clone Cocktail PAN-EpCAM]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

Western Blot analysis of human lung tissue lysate using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (PAN-EpCAM). Confirmation of Integrity and Purity of Antibody

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

Western Blot analysis of human lung tissue lysate using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (PAN-EpCAM). Confirmation of Integrity and Purity of Antibody

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1

Positive Control

MCF-7 or HT29 cells. Human breast tumor.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human EpCAM protein (full-length and fragments)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1 s, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or EpCAM. EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.