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Home >> Antibodies >> Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Mouse Monoclonal Antibody [Clone Ber-EP4]

Catalog #

Size

0.1 ml
0.5 ml

Price (USD)

199.00
429

Formulation

Culture Supernatant
Culture Supernatant

Catalog No

Size

0.1 ml
0.5 ml

Formulation

Culture Supernatant
Culture Supernatant

Price (USD)

199.00
429
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Ep-CAM Mouse Monoclonal Antibody (Ber-EP4)

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Ep-CAM Mouse Monoclonal Antibody (Ber-EP4)

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Flow Cytometric Analysis of PFA fixed MCF-7 cells using Ep-CAM Mouse Monoclonal Antibody (Ber-EP4) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1

Positive Control

MCF-7 or HT29 cells. Breast tumor.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human breast cancer MCF-7 cells

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Related Products



Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.

Key References

  1. Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol 1990;43:213-9.

Bioinformatics

  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2q21
  •  Unigene: 542050
NBT00825