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Home >> Antibodies >> EGFR (Epidermal Growth Factor Receptor)

EGFR (Epidermal Growth Factor Receptor)

Mouse Monoclonal Antibody [Clone H9B4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (H9B4). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (H9B4)

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Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).

Product Details

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Positive Control

A431 cells, Breast or Bladder cancer.

Known Applications & Suggested Dilutions

IP, Western Blot         More Details

Immunoprecipitation (1-2ug/500ug protein lysate)
Western Blot (1-2ug/ml for 2 hours at RT)
Optimal dilution for a specific application should be determined.

Immunogen

Purified EGFR from A431 cells.

Cellular Localization

Cell surface

Species Reactivity

Human. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~170kDa (wild type) and ~145kDa (vIII variant)

Specificity & Comments

This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin epiregulin.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bjorge JD et. al. Journal of Biological Chemistry, 1987, 262(14):6615-22.

Bioinformatics

  •  Entrez Gene ID: 1956
  •  Gene Symbol: EGFR
  •  SwissProt: P00533
  •  Chromosome Location: 7p11.2
  •  Unigene: 488293
NBT00642