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Home >> Antibodies >> EGFR (Epidermal Growth Factor Receptor)

EGFR (Epidermal Growth Factor Receptor)

Mouse Monoclonal Antibody [Clone GFR450]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

429.00
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Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled EGFR Monoclonal Antibody (GFR450).

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Flow Cytometry of EGFR on Mouse NIH/3T3 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled EGFR Monoclonal Antibody (GFR450).

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Confocal Immunofluorescent analysis of A431 cells using CF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).

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Confocal Immunofluorescent analysis of A431 cells using CF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Product Details

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Positive Control

A431 cells, Breast or Bladder cancer.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant extracellular domain of human EGFR protein

Cellular Localization

Cell surface

Species Reactivity

Human. Does not react with Mouse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

~170kDa (wild type) and ~145kDa (vIII variant)

Specificity & Comments

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

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Key References

  1. Tungekar MF et. al. Journal of Clinical Pathology. 51: 583-587 (1998).

Bioinformatics

  •  Entrez Gene ID: 1956
  •  Gene Symbol: EGFR
  •  SwissProt: P00533
  •  Chromosome Location: 7p11.2
  •  Unigene: 488293
NBT00450