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Home >> Antibodies >> EGFR (Epidermal Growth Factor Receptor)

EGFR (Epidermal Growth Factor Receptor)

Mouse Monoclonal Antibody [Clone GFR450]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/450). Confirmation of Integrity and Purity of Antibody.

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Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR Mouse Monoclonal Antibody (GFR450).

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Confocal Immunofluorescent analysis of A431 cells using AF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).

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Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (GFR/450)

Product Details

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Positive Control

A431 cells, Breast or Bladder cancer.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant extracellular domain of human EGFR protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Does not react with Mouse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

~170kDa (wild type) and ~145kDa (vIII variant)

Specificity & Comments

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Key References

  1. Tungekar MF et. al. Journal of Clinical Pathology. 51: 583-587 (1998).

Bioinformatics

  •  Entrez Gene ID: 1956
  •  Gene Symbol: EGFR
  •  SwissProt: P00533
  •  Chromosome Location: 7p11.2
  •  Unigene: 488293
NBT00450