SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/450). Confirmation of Integrity and Purity of Antibody.
Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR Mouse Monoclonal Antibody (GFR450).
Confocal Immunofluorescent analysis of A431 cells using AF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).
Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)
Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (GFR/450)
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.