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Home >> Antibodies >> EGFR (Epidermal Growth Factor Receptor)

EGFR (Epidermal Growth Factor Receptor)

Mouse Monoclonal Antibody [Clone GFR/2596]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human lung SqCC stained with EGFR Mouse Monoclonal Antibody (GFR/2596).

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Formalin-fixed, paraffin-embedded human placenta stained with EGFR Mouse Monoclonal Antibody (GFR/2596).

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SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (GFR/2596).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (GFR/2596)

Product Details

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Positive Control

A431 cells. Human placenta, breast, colon or bladder carcinoma tissue.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human EGFR protein (around aa 344-492) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

170kDa

Specificity & Comments

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Key References

  1. Tungekar MF et. al. Journal of Clinical Pathology. 51: 583 587 (1998).

Bioinformatics

  •  Entrez Gene ID: 1956
  •  Gene Symbol: EGFR
  •  SwissProt: P00533
  •  Chromosome Location: 7p11.2
  •  Unigene: 488293