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Home >> Antibodies >> E-Cadherin(CDH1) / CD324 (Intercellular Junction Marker)

E-Cadherin(CDH1) / CD324 (Intercellular Junction Marker)

Recombinant Rabbit Monoclonal Antibody [Clone CDH1/2208R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human cervix stained with E-CadherinRabbit RecombinantMonoclonal Antibody (CDH1/2208R).

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Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R).

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SDS-PAGE Analysis Purified E-Cadherin Rabbit RecombinantMonoclonal (CDH1/2208R). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of MCF-7cells labeling E-Cadherin with E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R) followed bygoat anti-mouse IgG-CF488. The nuclear counterstain is RedDot.

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Flow Cytometric Analysis of MCF-7cells using E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)

Positive Control

MCF-7 or SK-BR3 cells.Prostate or colon carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot(1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human E-Cadherin protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

120-80kDa (Mature); 135kDa (Precursor)

Specificity & Comments

Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- andP-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function.E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Umbas, R., et al. 1992. Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52: 5104-5109.

Bioinformatics

  •  Entrez Gene ID: 999
  •  Gene Symbol: CDH1
  •  SwissProt: P12830
  •  Chromosome Location: 16q22.1
  •  Unigene: 461086