Formalin-fixed, paraffin-embedded human cervix stained with E-CadherinRabbit RecombinantMonoclonal Antibody (CDH1/2208R).
Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R).
SDS-PAGE Analysis Purified E-Cadherin Rabbit RecombinantMonoclonal (CDH1/2208R). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of MCF-7cells labeling E-Cadherin with E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R) followed bygoat anti-mouse IgG-CF488. The nuclear counterstain is RedDot.
Flow Cytometric Analysis of MCF-7cells using E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- andP-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function.E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.