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Home >> Antibodies >> E2F6

E2F6

Mouse Monoclonal Antibody [Clone PCRP-E2F6-1F8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of HeLa cells using E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8) followed by goat anti-mouse IgG-CF488 (green). Phalloidin (red).

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SDS-PAGE Analysis Purified E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8).

Product Details

Synonyms

EMA; MGC111545

Positive Control

HeLa or Jurkat cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human E2F6 protein

Cellular Localization

Nucleus

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

35kDa

Specificity & Comments

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 1876
  •  Gene Symbol: E2F6
  •  SwissProt: O75461
  •  Chromosome Location: 2p25.1
  •  Unigene: 603093