Home >> Antibodies >> Desmoglein-3 (Squamous Cell Marker)

Desmoglein-3 (Squamous Cell Marker)

Mouse Monoclonal Antibody [Clone 5G11]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

459.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified Desmoglein-3 Monoclonal Antibody (5G11). Confirmation of Integrity and Purity of Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).

SDS-PAGE Analysis Purified Desmoglein-3 Monoclonal Antibody (5G11). Confirmation of Integrity and Purity of Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).

Product Details

Synonyms

130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Positive Control

A431 cells. Lung squamous cell carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human DSG3 extracellular protein fragment

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

130kDa

Specificity & Comments

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.