2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Desmoglein-3 (Squamous Cell Marker)

Desmoglein-3 (Squamous Cell Marker)

Mouse Monoclonal Antibody [Clone 5G11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

SDS-PAGE Analysis Purified Desmoglein-3 Monoclonal Antibody (5G11). Confirmation of Integrity and Purity of Antibody

img

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).

Product Details

Synonyms

130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Positive Control

A431 cells. Lung squamous cell carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human DSG3 extracellular protein fragment

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

130kDa

Specificity & Comments

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Mahoney, MG; Hu, Y; Brennan, D; Bazzi, H; Christiano, AM; Wahl, JK III. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol. 2006, 15(2):101-9.
  2. Wahl JK III. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21(1):37-44.

Bioinformatics

  •  Entrez Gene ID: 1830
  •  Gene Symbol: DSG3
  •  SwissProt: P32926
  •  Chromosome Location: 18q12.1
  •  Unigene: 1925
NBT01439