SDS-PAGE Analysis Purified DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of PFA-fixed HeLa cells. DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescent staining of PFA-fixed human cell line MCF7. DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3'exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay