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Home >> Antibodies >> DCP2(Decapping mRNA 2)

DCP2(Decapping mRNA 2)

MouseMonoclonal Antibody [Clone PCRP-DCP2-1D6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6). Confirmation of Purity and Integrity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

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Immunofluorescent staining of PFA-fixed human cell line MCF7. DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6).

Product Details

Synonyms

DCP2 decapping enzyme homolog (S. cerevisiae); DFLJ33245; hDpc; m7GpppN-mRNA hydrolase; mRNA decapping enzyme 2; nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20)

Positive Control

MCF7, Daudi or Jurkatcells.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human DCP2protein

Cellular Localization

Cytoplasm > P. body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.

Species Reactivity

Human. Predicted to react with Mouse and Rat.

Host / Ig Isotype

Mouse/ IgG2a

Mol. Weight of Antigen

48.4kDa

Specificity & Comments

The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3'exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wang, Z., et al. 2002. Proc. Natl. Acad. Sci. USA 99: 12663-12668.

Bioinformatics

  •  Entrez Gene ID: 167227
  •  Gene Symbol: DCP2
  •  SwissProt: Q8IU60
  •  Chromosome Location: 5q22.2
  •  Unigene: 443875