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Home >> Antibodies >> Cytokeratin, pan (Epithelial Marker)

Cytokeratin, pan (Epithelial Marker)

Mouse Monoclonal Antibody [Clone SPM115 + SPM116]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to PE

Price (USD)

429.00
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Flow Cytometric analysis of human Pan-Cytokeratins on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (SPM115 + SPM116).

Product Details

Synonyms

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Positive Control

Skin, Adeno- or Squamous carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100 for 30 minutes at RT)
Optimal dilution for a specific application should be determined.

Immunogen

Human epidermal keratin

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)

Specificity & Comments

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This MAb is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  2. Tseng SCG et. al. Cell 1982; 30361.

Bioinformatics

NBT00371