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Home >> Antibodies >> Cytokeratin, pan (Epithelial Marker)

Cytokeratin, pan (Epithelial Marker)

Mouse Monoclonal Antibody [Clone PCK/3150]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150).

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Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150).

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Immunofluorescence staining of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nucleus stained with Reddot (Red).

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SDS-PAGE Analysis Purified Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Positive Control

Skin, adeno- or squamous carcinomas (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Dog. Cat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

40-67kDa (Multiple)

Specificity & Comments

Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Battifora H, Am J Surg Pathol. 1988;12:24
  2. Gown AM, et al. Am J Clin Pathol. 1985;84:413

Bioinformatics

  •  Entrez Gene ID: Multiple
  •  Gene Symbol: KRT
  •  SwissProt: Multiple
  •  Chromosome Location: Multiple
  •  Unigene: Multiple
NBT03150