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Home >> Antibodies >> Cytokeratin, pan (Epithelial Marker)

Cytokeratin, pan (Epithelial Marker)

Mouse Monoclonal Antibody [Clone Cocktail PAN-CK]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK).

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Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK).

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Confocal immunofluorescent analysis of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).

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Flow cytometric analysis of human HeLa cells. Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Positive Control

HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (0.5-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-2ug/ml for 2 hours at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human epidermal keratins

Cellular Localization

Cytoplasmic

Species Reactivity

Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Cat and Chicken.

Host / Ig Isotype

Mouse / IgG s, kappa

Mol. Weight of Antigen

40-67kDa (Multiple)

Specificity & Comments

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  2. Tseng SCG et. al. Cell 1982; 30361.

Bioinformatics