Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK).
Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK).
Confocal immunofluorescent analysis of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).
Flow cytometric analysis of human HeLa cells. Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.