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Home >> Antibodies >> Cytokeratin, Basic (Type II or HMW)

Cytokeratin, Basic (Type II or HMW)

Recombinant Rabbit Monoclonal Antibody [Clone KRTH/2147R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CK HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R).

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SDS-PAGE Analysis of Purified CK HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with CK HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Product Details

Synonyms

KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

Positive Control

MCF-7, PC12 cells, Skin, Prostate carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2 ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Solubilized keratin extract from human stratum corneum

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

50-67kDa

Specificity & Comments

This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
  2. Varma M et. al. Mod Pathol 1999;12(5):472-8

Bioinformatics

  •  Entrez Gene ID: 51350
  •  Gene Symbol: KRT76
  •  SwissProt: Q01546
  •  Chromosome Location: 12q13.13
  •  Unigene: 654392
NBT02147