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Home >> Antibodies >> Cytokeratin, Basic (Type II or HMW)

Cytokeratin, Basic (Type II or HMW)

Mouse Monoclonal Antibody [Clone DE-SQ]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin, HMW Mouse Monoclonal Antibody (DE-SQ).

Product Details

Synonyms

KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

Positive Control

PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

50-67kDa

Specificity & Comments

This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
  2. Varma M et. al. Mod Pathol 1999;12(5):472-8

Bioinformatics

  •  Entrez Gene ID: 51350
  •  Gene Symbol: KRT76
  •  SwissProt: Q01546
  •  Chromosome Location: 12q13.13
  •  Unigene: 654392
NBT00860