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Home >> Antibodies >> Cytokeratin 8/18

Cytokeratin 8/18

Mouse Monoclonal Antibody [Clone 5D3]

Catalog #

Size

0.1 ml
0.5 ml

Price (USD)

199.00
429

Formulation

Culture Supernatant
Culture Supernatant

Catalog No

Size

0.1 ml
0.5 ml

Formulation

Culture Supernatant
Culture Supernatant

Price (USD)

199.00
429
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (5D3).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (5D3).

Product Details

Synonyms

CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Positive Control

MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (2-5ul/million cells)
Immunofluorescence (1:100-1:200)
Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Cytoskeleton preparation from human breast cancer MCF-7 cells

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

52.5kDa (CK8); 45kDa (CK18)

Specificity & Comments

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

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Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.

Key References

  1. Bartek et al. J. Pathology,164:215-24 (1991).
  2. Bartkova et al. Neoplasma 38(4), 439-46 (1991)

Bioinformatics

NBT01458