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Home >> Antibodies >> Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone KRT7/1499R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Oviduct stained with Cytokeratin 7 Rabbit Monoclonal Antibody (KRT7/1499R).

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Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Rabbit Monoclonal Antibody (KRT7/1499R).

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SDS-PAGE Analysis Purified Cytokeratin 7 Rabbit Recombinant Monoclonal Antibody (KRT7/1499R).

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Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 7 Rabbit Recombinant MAb (KRT7/1499R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red)

Product Details

Synonyms

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Positive Control

HeLa cells. Carcinoma of ovary, lung, cervix or breast.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT7 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

55kDa

Specificity & Comments

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Related Products



200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990;136:641-55

Bioinformatics

  •  Entrez Gene ID: 3855
  •  Gene Symbol: KRT7
  •  SwissProt: P08729
  •  Chromosome Location: 12q13.13
  •  Unigene: 411501
NBT01499