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Home >> Antibodies >> Cytokeratin 7/17

Cytokeratin 7/17

Mouse Monoclonal Antibody [Clone C-46]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7/17 Mouse Monoclonal Antibody (C-46).

img

SDS-PAGE Analysis Purified Cytokeratin 7/17 Mouse Monoclonal Antibody (C-46). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7; CK17; K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1

Positive Control

HeLa or ME-180 cells. Skin or hepatocellular carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Cow. Does not react with Mouse, Rat, Ferret or Rabbit.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55kDa (CK7); 46kDa (CK17)

Specificity & Comments

It recognizes intermediate filament proteins (IFP's) of 55kDa and 46kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  2. Vojtesek B, Staskova Z, Nenutil R, Bartkova J, Kovarik J, Rejthar A, Bartek J: A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 1990;37(3):333-42.
  3. Bartek J et. al. Journal of Pathology, 1991, 164(3): 215-24.

Bioinformatics

NBT00949