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Home >> Antibodies >> Cytokeratin 5/8

Cytokeratin 5/8

Mouse Monoclonal Antibody [Clone C-50]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Cytokeratin 5/8 Monoclonal Antibody (C-50). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (C-50).

Product Details

Synonyms

CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8

Positive Control

HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Immunogen

Cytoskeletal preparation from HeLa cells

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

58kDa (CK5); 52.5kDa (CK8)

Specificity & Comments

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

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0.5 ml at 200 ?g/ml of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.

Key References

  1. Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  2. Vojtek et al. Folia Biol. 37(3-4): 197-206 (1991).
  3. Bartek et al. J. of Pathology 164, 215-24 (1991).
  4. Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).

Bioinformatics

NBT00948