Home >> Antibodies >> Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Mouse Monoclonal Antibody [Clone SPM191]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (SPM191).

SDS-PAGE Analysis Purified Cytokeratin 20 Mouse Monoclonal Antibody (SPM191). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of human HT29 cell lysate using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (SPM191).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (SPM191).

SDS-PAGE Analysis Purified Cytokeratin 20 Mouse Monoclonal Antibody (SPM191). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of human HT29 cell lysate using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (SPM191).

Product Details

Synonyms

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Positive Control

HT29 cells. Colon Carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.