Home >> Antibodies >> Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone KRT20/7805R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/7805R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/7805R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Positive Control

HT29 cells. Human colon or prostate carcinoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human KRT20 protein (around aa aa324‐424) (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. �It has been detected in adenocarcinomas of the colon, stomach �and biliary tract. � Breast carcinomas are generally non-reactive.