Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 18 Mouse Monoclonal Antibody (SPM265).
Western Blot 1) HeLa and 2) A431 lysate using Cytokeratin 18 Mouse Monoclonal Antibody (SPM265).
Confocal Immunofluorescence of HeLa cells Cytokeratin 18 Mouse Monoclonal Antibody (SPM265). labeled with CF488 (Green); Reddot is used to label the nuclei.
Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (SPM265).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.