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Home >> Antibodies >> Cytokeratin 18 (KRT18)

Cytokeratin 18 (KRT18)

Recombinant Rabbit Monoclonal Antibody [Clone KRT18/2808R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).

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SDS-PAGE Analysis Purified CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human HCT116 cell lysate using CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R).

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Immunofluorescence Analysis of MCF-7 cells labeling CK18 with CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Flow Cytometric Analysis of HeLa cells using CK18 Rabbit Recombinant Monoclonal Antibody (KRT18/2808R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Positive Control

HCT116, MCF-7, HeLa, A431 cells. Breast Cancer.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length KRT18 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

45kDa

Specificity & Comments

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lauerova, L Hybridoma, 7, 495-504 (1988).
  2. Lane EB, Alexander CM. Sem Cancer Biol 1:165-179 (1990).

Bioinformatics

  •  Entrez Gene ID: 3875
  •  Gene Symbol: KRT18
  •  SwissProt: P05783
  •  Chromosome Location: 12q13.13
  •  Unigene: 406013
NBT02808