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Home >> Antibodies >> Cytokeratin 18 (KRT18)

Cytokeratin 18 (KRT18)

Mouse Monoclonal Antibody [Clone DE-K18]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Mouse Monoclonal Antibody (DE-K18).

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Western Blot Analysis of MCF-7cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (DE-K18).

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SDS-PAGE Analysis Purified Cytokeratin 18 Mouse Monoclonal Antibody (DE-K18). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (DE-K18).

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Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Mouse Monoclonal Antibody (DE-K18) Green (CF488) and Reddot is used to label the nuclei.

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Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using Cytokeratin 18 Mouse Monoclonal Antibody (DE-K18) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Positive Control

MCF-7, HeLa cells, Breast Cancer.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Dog. Cat. Does not react with Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ivanyi D, et. al. Journal of Pathology, 1989, 159:7-12.
  2. Ivanyi D, et. al. Differentiation, 1989, 42(2):124-9.

Bioinformatics

  •  Entrez Gene ID: 3875
  •  Gene Symbol: KRT18
  •  SwissProt: P05783
  •  Chromosome Location: 12q13.13
  •  Unigene: 406013
NBT00563