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Home >> Antibodies >> Cytokeratin 17 (KRT17) (Basal Epithelial Marker)

Cytokeratin 17 (KRT17) (Basal Epithelial Marker)

Mouse Monoclonal Antibody [Clone SPM560]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human skin stained with CK17 Monoclonal Antibody (SPM560).

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Formalin-fixed, paraffin-embedded human skin stained with CK17 Monoclonal Antibody (SPM560).

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Western blot of HeLa lysate using CK17 Monoclonal Antibody (SPM560).

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Flow Cytometric Analysis of PFA-fixed HeLa cells labeling CK17 with CK17 MAb (SPM560) followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

Product Details

Synonyms

K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1

Positive Control

HeLa cells, Skin.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

The cytoskeletal fraction of rat colon epithelium

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Rat. Cow. Goat. Pig.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFP's). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glandssuch as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as 'epithelial stem cell' marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK56, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Smedts et. al. Am J Pathol 141: 497, 1992.
  2. Smedts et. al. Am J Pathol 140: 601, 1992.
  3. Wetzels et. al. Histopathol 20: 295, 1992.

Bioinformatics

  •  Entrez Gene ID: 3872
  •  Gene Symbol: KRT17
  •  SwissProt: Q04695
  •  Chromosome Location: 17q21.2
  •  Unigene: 2785
NBT00158