Home >> Antibodies >> Cytokeratin 14 (KRT14) (Squamous Cell Marker)

Cytokeratin 14 (KRT14) (Squamous Cell Marker)

Biotinylated Mouse Monoclonal Antibody [Clone LL002]

Printable

Key References

Bioinformatics

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

499.00

Formulation

Purified Ab conjugated to Biotin

Performance Guarantee

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to Biotin

Price (USD)

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human skin stained with Biotin-conjugated Cytokeratin 14 Mouse Monoclonal Antibody (LL002).

Formalin-fixed, paraffin-embedded human skin stained with Biotin-conjugated Cytokeratin 14 Mouse Monoclonal Antibody (LL002).

SDS-PAGE Analysis Purified Cytokeratin 14 Mouse Monoclonal Antibody (LL002) (unconjugated). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human skin stained with Biotin-conjugated Cytokeratin 14 Mouse Monoclonal Antibody (LL002).

SDS-PAGE Analysis Purified Cytokeratin 14 Mouse Monoclonal Antibody (LL002) (unconjugated). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; Keratin Type I Cytoskeletal 14; Koebner; NFJ

Positive Control

HeLa, A549, or A431 cells. Skin or squamous cell carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (2-4ug/million cells)
Immunofluorescence (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °
C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.

Cellular Localization

Cytoplasmic

Species Reactivity

Human, Mouse and Rat.

Host / Ig Isotype

Mouse / IgG3, kappa

Mol. Weight of Antigen

50kDa

Specificity & Comments

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.