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Home >> Antibodies >> Cytokeratin 14 (KRT14) (Squamous Cell Marker)

Cytokeratin 14 (KRT14) (Squamous Cell Marker)

Mouse Monoclonal Antibody [Clone KRT14/2375]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).

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Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).

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SDS-PAGE Analysis Purified Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of HeLa cells using Cytokeratin 14 Mouse Monoclonal Antibody ((KRT14/2375) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).

Product Details

Synonyms

CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; k14; Keratin 14; Keratin Type I Cytoskeletal 14; Koebner; krt14; NFJ

Positive Control

A431 or HeLa cells. Skin or Squamous Cell Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human KRT14 fragment (around aa351-472) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Pig.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

50kDa

Specificity & Comments

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Leigh I.M., et al. (1988) Dermatology, 91:415.
  2. P.Purkis et al. 1990. Journal of Cell Science. 97: p39-50.

Bioinformatics

  •  Entrez Gene ID: 3861
  •  Gene Symbol: KRT14
  •  SwissProt: P02533
  •  Chromosome Location: 17q21.2
  •  Unigene: 6545380
NBT02375