Home >> Antibodies >> Cytokeratin 10 (Suprabasal Epithelial Marker)

Cytokeratin 10 (Suprabasal Epithelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rKRT10/6923]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified KRT10 Recombinant Mouse Monoclonal Antibody (rKRT10/6923). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human skin stained with KRT10 Recombinant Mouse Monoclonal Antibody (rKRT10/6923). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified KRT10 Recombinant Mouse Monoclonal Antibody (rKRT10/6923). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human skin stained with KRT10 Recombinant Mouse Monoclonal Antibody (rKRT10/6923). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Positive Control

Human esophagus or tonsil.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa384-584) of human KRT10 protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

56.5kDa

Specificity & Comments

This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.