Home >> Antibodies >> Cycloxygenase-2 (COX-2)

Cycloxygenase-2 (COX-2)

Mouse Monoclonal Antibody [Clone rCOX2/6996]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with COX-2 Mouse Monoclonal Antibody (rCOX2/6996). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified COX-2 Mouse Monoclonal Antibody (rCOX2/6996). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with COX-2 Mouse Monoclonal Antibody (rCOX2/6996). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified COX-2 Mouse Monoclonal Antibody (rCOX2/6996). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; Macrophage activation-associated marker protein P71/73; PES-2; PGG/HS; PGHS-2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin endoperoxide synthase 2; PTGS2; TIS10

Positive Control

Human colon or lung carcinoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human COX2 protein fragment (around aa 504-604) (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70-72kDa

Specificity & Comments

Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90% of human colorectal adenocarcinomas whereas levels of COX-1 are not changed.