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Home >> Antibodies >> Cyclin D1 (G1-Cyclin & Mantle Cell Marker)

Cyclin D1 (G1-Cyclin & Mantle Cell Marker)

Mouse Monoclonal Antibody [Clone DCS-6]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to PE

Price (USD)

429.00
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Flow Cytometric analysis of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).

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Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).

Product Details

Synonyms

B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

Positive Control

T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60% of mantle cell lymphomas and ~40% of breast carcinomas are positive.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100 for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Immunogen

Human recombinant full length cyclin D1 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Monkey. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Lukas J, et. al. Oncogene, 1994, 9(3):707-18.
  2. Gillett C, et. al. Cancer Research, 1994, 54(7):1812-7.
  3. Bartkova J, et. al. Journal of Pathology, 1994, 172(3):237-45.

Bioinformatics

  •  Entrez Gene ID: 595
  •  Gene Symbol: CCND1
  •  SwissProt: P24385
  •  Chromosome Location: 11q13.2
  •  Unigene: 523852, 667996
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