2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Cyclin D1 (G1-Cyclin & Mantle Cell Marker)

Cyclin D1 (G1-Cyclin & Mantle Cell Marker)

Mouse Monoclonal Antibody [Clone CCND1/809]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

img

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

img

Flow Cytometric analysis of human Cyclin D1 on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

img

Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

img

Purified Cyclin D1 Mouse Monoclonal Antibody (CCND1/809). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

Positive Control

T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CCND1 protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Baldin V; Lukas J; Marcote MJ; Pagano M; Draetta G. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes and Development, 1993, 7(5):812-21.

Bioinformatics

  •  Entrez Gene ID: 595
  •  Gene Symbol: CCND1
  •  SwissProt: P24385
  •  Chromosome Location: 11q13.2
  •  Unigene: 523852, 667996
NBT00809